Werkervaringen, technieken

  • 04-2018 / 08-2018
    Research assistant at the laboratory of Prof. L. Kauppi, genome stability group in the Reseach Program Systems Oncology (ONCOSYS) at the University of Helsinki. In this group we developed an ex vivo protocol that predicts response to chemotherapy in ovarian cancer patients and that can allow us to identify new ways to exploit DNA repair deficiencies in chemoresistant en recurrent tumors.
  • 03-2017 / 03-2018
    Research assistant at the laboratory of Prof H. Jacobs under supervision of K. Holmström in Viikki campus of the University of Helsinki. Prof. Jacobs’ group recently generated mouse models that express the Ciona instestinalis alternative oxidase (AOX) and alternative NADH dehydrogenase (NDX). These models provide a unique opportunity to examine the potential of this novel approach in fighting neurodegeneration. In this group I am lab managing and participating in investigation the role of AOX and NDX in neurodegenerative diseases
  • 12-2013 / 12-2014
    Bioanalyst at the laboratory of Prof. K. Kaila in Viikki campus of The University of Helsinki. In this group I am responsible for general lab management, several mouse strains (breeding, genotyping), executing different molecular biological experiments.
  • 02-2009 / 11-2013
    Labtechnician at the laboratory of Dr. E. Verschuren and Dr. S. Kuznetsov in the Institute for Molecular Medicine Finland (FIMM), University of Helsinki, Finland. In this group I was lab managing and participated in investigating the role of Rad51c in cancer.
  • 10.2002 / 10.2008 – 02-2002 / 06-2002
    Bioanalyst/ master undergraduate tranining at the laboratory of Prof P. Ojala and Prof T. Mäkelä in Biomedicum, Helsinki, Finland. The main focus was on v-cyclin a cyclin-D homolog and it role KSHV associated tumorigenesis. Where we studied the consequences of expression of v-cyclin in primary and immortalized human dermal microvascular endothelial cells.
    Undergraduate training at VTT technical research centre of Finland, protein engineering group, supervisor Dr. H. Boer and Dr. A. Koivula. In this group I participated in the purification and mutagenesis of two chitinases, Chit33 and Chit42, from the filamentous fungus Trichoderma harzianum. We were particularly interested in the potential of these enzymes in recognizing certain animal-type saccharides of medical relevance.
  • 09.2001 / 06.2002
    Undergraduate training for B.Sc degree at the university of Groningen, faculty of medical science, group stem cell biology, Prof. G de Haan and supervisor Dr. E.J.K Noach. In this training I got extensive hands-on experience with mouse models. We assessed how bone marrow transplantation in mice was affected by prior chemotherapy since it has been reported that lymphoma patients treated with intensified cycles of chemotherapy followed by autologous bone marrow transplantation have a poor hematopoietic reconstitution.

TECHNICAL SKILLS

  • Common methods of molecular biology
  • Extensive cell culture (primary cells, patient material, cancer cell lines)
  • Patient material (ovarian cancer)
  • Western blot (x-ray and odyssey)
  • PCR, (genotyping, cloning, )
  • Microscopy (including imaging)
  • Oxygraph measurements
  • Immunocytochemistry
  • RNA interference techniques (RNAi)
  • Retroviral/Lentiviral based shRNA techniques
  • Histology both cutting and staining (paraffin and cryosections)
  • Mice (Artikel 12 accomplished), handling/breeding, genotyping, perfusions, dissecting
  • Flow cytometric analysis
  • Proliferation assays / Soft agar
  • In vitro kinase assays
  • Lab management (ordering (quartzy), Ivoice-checking (SAP-system)

Cursussen, trainingen

Proefdier diploma artikel 12 behaald.

Diversen, hobbies

Hardlopen, wandelen met de hond, lezen

Wensen t.a.v. nieuwe functie

  • Research analist, medisch analist, microbiologisch analist, research assistant
  • Voorkeur gaat uit naar 24-32 uur per werkweek.